A group from the Karolinska Institute in Stockholm (SE) performed a genomic sequencing study of the coronavirus, was conducted using a method called CUTseq (Zhang X. et al. 2019), which is a versatile technique for preparing multiplex DNA sequencing libraries from low-input samples. The research found that CUTseq can be easily applied for ongoing pandemic genomic surveillance and adapted to other pathogens such as influenza viruses. Additionally, a cost analysis showed that COVseq, based on CUTseq, could be used to sequence thousands of samples per week at less than $10 USD per sample, including library preparation and sequencing costs.In this blog post, we describe briefly how the Karolinska Institute research group of Nicola Crosetto, PhD and MD, utilized Immediate Drop-on-demand Technology (I.DOT) to automate and optimize the COVseq workflow with high precision, speed and significant reduction of liquid reagent volumes. Since the identification of SARS-CoV-2, thousands of virus genomes have been sequenced worldwide, and the sequences have been made publicly available. This scientific movement has enabled a phylogenetic reconstruction of viral spreading and evolution in different countries and continents, allowing the rapid identification of genomic variations of potential epidemiological concern.
SARS-CoV-2 whole genome sequencing (WGS) is increasingly being applied in epidemiological surveillance to track infections in hospitals and other communities, in order to inform public health decisions. In genomic surveillance, the availability of rapid and cost-effective methods for sequencing hundreds or even thousands of samples per week would be tremendously advantageous.
Therefore, in the research conducted by the team of Nicola Crosetto (Simonetti, 2021), they used a versatile method for preparing multiplex DNA sequencing libraries from low-input samples, called CUTseq (Zhang, 2019).
Their COVseq protocol, based on CUTseq, enabled the construction of highly multiplexed sequencing libraries starting with small volumes of purified RNA samples that, thanks to the I.DOT non-contact liquid handler, drastically reduced reagent volumes and therefore the cost per sample.
To get further details on the procedures, materials and methods involved in this study, read the full article here.
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These experiments have been made possible thanks to the use of the I.DOT non-contact liquid handler, a highly versatile benchtop instrument that requires minimal maintenance and training.
Learn more about the I.DOT and all of other innovative technologies for next-generation sequencing (NGS).