Did you know that liquid handling automation saves labs hours of hands-on time? Welcome back to episode three of “Library Prep with the I.DOT Liquid Handler.” Today, we're talking about the KAPA HyperPrep Library Prep Kit. If you use this in your lab, keep watching.
Here are some of the reasons why it's such a popular kit and look! It has qualified automation methods! KAPA is cool because while it does not supply liquid handling equipment, it does supply automation-friendly protocols, and that's where we come in.
The I.DOT Liquid Handler is a non-contact microfluidic dispenser that does not require any tips, training, or automation experience.
The I.DOT is designed to dispense up to 65% glycerol, so even the most viscous fragmentation enzymes can be dispensed using the I.DOT.
For library prep, use the I.DOT to dispense the End Repair & A-tailing Buffer and Enzyme, the ligation buffer, DNA ligase, and, obviously, your adapters of choice.
For post ligation processing, reaction clean-ups, and size selection, you can use the G.PURE NGS Clean-up Device. This can also dispense your ethanol and resuspension buffer. It's also completely tip-free and consumable-free, in general.
For library amplification, he I.DOT can dispense the HiFi HotStart ReadyMix and the Library Amplification Primer Mix.
Pool using the I.DOT and variable volumes of your library and backfilling with water, and that's it!
Automated library prep! So easy! Ready to automate your library prep? Book a demo today!