Optimizing high-throughput single-cell RNA-seq protocols as well as emerging single-cell proteomics and multi-omics assays
Falling under the umbrella of multi-omics, the equivalent of big data mining for biological systems, are approaches such as proteomics, transcriptomics, genomics, metabolomics, lipidomics and epigenomics, which analyze proteins, RNA, genes, metabolites, lipids or DNA, respectively. To develop more accurate (predictive or prognostic) molecular models for particular conditions and diseases, medical researchers are using the integrated approach of multi-omics to combine, compare and analyze data from these individual omics research areas. In order to do so, they need robust platforms to optimize plate-based single-cell workflows such as DNA or RNA sequencing.
How the I.DOT facilitates multi-omics
Dispendix’s portfolio includes the I.DOT featuring the patented Immediate Drop-on-demand Technology (I.DOT) that allows hundreds of libraries to be quantified and normalized within minutes by facilitating the highly multiplexed dispensing of up to 96 reagents in parallel.
Quantitation and Normalization of NGS libraries
Typically, cDNA amplification from single cells results in uneven concentrations, which may require normalization to ensure an even read distribution for all cells.
LIBRARY INDEXING AND EARLY BARCODING
The ability to index NGS libraries with unique DNA barcodes provides the basis for high-throughput single-cell sequencing. For single-cell full-length transcript sequencing or whole exome sequencing, individual libraries are usually indexed after amplification and fragmentation.
PERFORMING COMPLEX TASKS AT UNRIVALED SPEED
The I.DOT non-contact dispensing technology was developed to perform even the most complex liquid-handling tasks at unrivaled speeds. Up to 2304 libraries can be uniquely barcoded with a single I.DOT source plate. Dispensing 96 unique barcodes takes less than 60 seconds.
FROM GENOMICS TO TRANSCRIPTOMICS, PROTEOMICS AND MULTI-OMICS
From the DNA assembly (bioengineering or genetic engineering), whole genome amplification (WGA) and whole genome sequencing (WGS) the use of I.DOT technology for delivery is highly flexible and can dispense many different DNA fragments simultaneously with their respective amplification reagents. Focusing on proteome and protein quantification in single cells, the I.DOT is used in single cell proteomics for sample preparation for mass spectrometry. However, the main use of the I.DOT remains in RNA sequencing and transcriptomics where it is mainly used in NGS workflows.