NGS Library Preparation
From Purified DNA to High-quality NGS Libraries, DISPENDIX Has You Covered
Your Partner For Automated Next-Generation Sequencing
DISPENDIX excels in a wide range of sequencing approaches, empowering them to facilitate the development of highly robust and cost-efficient automated pipelines for Next Generation Sequencing (NGS).
DNA-Sequencing
From genome wide association studies in basic research to cfDNA analysis in clinical environments, DNA sequencing allows researchers to understand the genetic make-up of their sample.
RNA-Sequencing
In gene expression studies, RNA sequencing allows researchers to understand which genes are actively expressed in samples, providing deeper insights into disease pathways that can enable the development of novel therapeutics.
Targeted Sequencing
In clinical diagnostics environments, targeted sequencing allows for panels of mutations in genes to be detected at a fraction of the cost of whole genome analysis enabling the next wave of precision medicine.
I.DOT Liquid Handler
Every step in the NGS Library Preparation Process involves adding microliter scale volumes of reagents to nucleic acid samples. In fact, up to 80% of the library preparation process can be automated by a reagent dispenser.
At DISPENDIX, we have designed the perfect reagent dispenser for NGS. The I.DOT Liquid Handler allows researchers to dispense microliter scale volumes of enzymes, buffers, and beads to samples throughout the library preparation process with the click of a button.
Don't Take Our Word for It
Our customer Kevin Mohammed from the Mt. Sinai School of Medicine’s Center for Oncogenomic and Innovative Therapeutics has integrated the I.DOT into their precision oncology research. Watch the short talk below to witness a real-world example of how DISPENDIX products bridge the gap between medium- or low-throughput methods and high-throughput solutions, especially in academic research settings.
Efficiency and Cost Savings
The I.DOT significantly reduced manual effort and increased efficiency for tasks like loading 384-well qPCR plates and normalizing nucleic acids, cutting process times from hours to minutes. These productivity gains, along with cost savings on reagents and media, are key advantages for other researchers in research and clinical settings.
Innovative Use Cases
Kevin’s lab demonstrated how the I.DOT enables cutting-edge experiments like detecting oncopetal signatures in miniaturized 384-well organoid systems, proving that DISPENDIX technology supports sophisticated cancer biology research.
Application in Drug Discovery
By utilizing the I.DOT, the Guccione lab has miniaturized experiments such as IC50 and IC80 drug curves and successfully integrated it into their next-generation sequencing (NGS) workflows. This application can resonate with labs looking to scale up their drug discovery efforts while controlling costs and reducing sample volumes.
Collaborative Relationships
Kevin’s acknowledgment of DISPENDIX's support, from procurement to troubleshooting, shows the strength of DISPENDIX’s commitment to collaboration and partnership.
Fragmentation, Ligation, & Amplification
The core of the NGS workflow requires adding enzymes and buffers to nucleic acid samples. In the simplest case of DNA sequencing experiments, the I.DOT can be used to dispense the following library preparation reagents:
- Fragmentation Enzyme/Buffer
- Ligation Enzyme/Buffer
- Amplification Mastermix
Bead-Based Clean-Ups
Following steps like adapter ligation and PCR amplification, most SOPs include "DNA Clean-Ups" to remove undesirable adapters, primers, and dimers from each sample.
DNA Clean-ups accounts for the most error-prone and challenging steps of the NGS Library Preparation process to perform reliably. Further, NGS Clean-Ups have a direct impact on final NGS library quality and yield.
The G.PURE NGS Clean-Up Device automates the NGS clean-up process leading to increased library yield and quality for every sample.
Unlocking The Most Cost-Effective & Reproducible NGS Workflow
Although reaction miniaturization is ubiquitous in large pharmaceutical environments, it can be challenging to understand the risks and considerations associated with generating high-quality data in the context of miniaturizing assays.
The DISPENDIX team has worked through miniaturization of even the most complex NGS workflows, and can help you to scale volumes as low as 1/10th the manufacturers recommended SOP without compromising data quality.
Combinatorial Indexing
When it comes to barcoding your NGS libraries, manual pipetting can be a tedious and error prone approach. Since barcoding directly enables deconvolution of your NGS data, it is critical to get the correct index primer in the intended sample.
The I.DOT will not only dispense barcodes to the proper well. The I.DOT will count the droplets dispensed and provide a log file that provides evidence that the correct barcode was loaded into the correct wells of your microtiter plate.
Normalization & Pooling
Following library preparation, quality control analysis provides the concentration of each library individual NGS library. In the case where samples will be pooled for cost-effective sequencing, they must be normalized to the same concentration to avoid oversampling of one library relative to another.
The I.DOT can perform a normalization of up to 96 NGS libraries in minutes with a 1 μL dead volume per sample, providing a much more efficient solution than manual pipetting and traditional automated liquid handlers.
Further, the I.DOT can pool a variable volume of each sample into a single well allowing for normalization and pooling to occur in a single step - now that is efficiency!
The Newest In Full Workflow Automation for NGS
If your lab is tired of painstakingly preparing samples for sequencing, the G.STATION NGS Workstation enables researchers to run up to 96 samples through a DNA sequencing pipeline free from any manual intervention.