Design of Experiments for Assay Development and Optimization
Design of experiments (DoE) for drug discovery assays is a statistical and systematic approach used to optimize the experimental conditions and parameters in the drug discovery process. It involves strategically planning and conducting experiments to understand the relationship between multiple variables and their impact on the assay outcome. DoE helps identify critical factors and their optimal settings, allowing researchers to maximize the efficiency, reliability, and effectiveness of drug discovery assays. By using DoE methodologies, scientists can minimize experimental variability, reduce costs, and accelerate the development of new drugs. It plays a crucial role in optimizing assay conditions and improving the success rate of drug discovery efforts.
I.DOT Liquid Handling
The I.DOT liquid handler is a valuable tool that can support the Design of Experiments (DoE) process in drug discovery assays. It enables precise and controlled dispensing of small volumes of assay components without physical contact, minimizing the risk of contamination and improving experimental accuracy.
With the I.DOT dispenser, researchers can easily create gradients of concentrations and volumes of assay components, allowing them to systematically explore the impact of different variables on assay outcomes. This automated dispenser streamlines the DoE process by providing reliable and consistent dispensing, facilitating the optimization of experimental conditions and enhancing the efficiency and reproducibility of drug discovery assays.
Hear Directly from Our Customers
Enhanced Throughput & Efficiency
The integration of I.DOT significantly increased the number of compounds tested per week by eliminating time-consuming manual processes. This streamlined workflow allows for faster data generation and decision-making in drug screening.
Improved Reproducibility & Data Quality
By automating liquid handling, I.DOT minimizes human variability, ensuring precise and consistent drug dosing. This has led to more reliable cytotoxicity assay results, reducing the risk of inconsistencies due to manual pipetting errors.
Less Manual Intervention & Cost Savings
I.DOT eliminates the need for serial dilutions—reducing reagent waste, cross-contamination risk, and operator-dependent variability. This not only improves cost efficiency but also frees up researchers' time for higher-value tasks.
Scalability & Future Innovation
The lab plans to expand I.DOT’s use to additional assays, integrate it with robotic systems for further automation, and miniaturize experiments to enhance efficiency. These advancements will further optimize workflows and accelerate drug discovery.
Precision in Low-Volume Liquid Handling
The I.DOT enabled highly accurate and reproducible dispensing of nanoliter to microliter volumes, which was crucial for reliable drug screening.
Increased Efficiency in Drug Screening
By automating the preparation of antibiotic combinations, the I.DOT streamlined high-throughput screening, accelerating the discovery of synergistic drug interactions.
Minimization of Reagent Waste
The non-contact dispensing technology reduced dead volumes, minimizing waste of expensive antibiotics and making the workflow more cost-effective.
Enhanced Reproducibility
The automated dispensing process significantly reduced human error, ensuring consistent and reliable experimental results.