Dispendix - Blog

Automate Your Way to NGS Success: The Power of I.DOT Liquid Handler & G.PURE Clean-Up Device

Written by Petros Apostolopoulos | Mar 12, 2024 4:31:39 PM

NGS Workflow Overview

 

Every step in the NGS (next-generation sequencing) library preparation process involves adding microliter scale volumes of reagents to nucleic acid samples. In fact, up to 80% of the library preparation process can be automated by a reagent dispenser.

At DISPENDIX, we have designed the perfect reagent dispenser for NGS. The I.DOT Liquid Handler allows researchers to dispense microliter scale volumes of enzymes, buffers, and beads to samples throughout the library preparation process with the click of a button (Fig. 1).

Figure 1. Workflow schematic showing where the I.DOT Liquid Handler and G.PURE Clean-up Device fit into the NGS library preparation workflow.

For clean-up steps in the process, The G.PURE Clean-Up Device is a device designed to automate bead-based clean-ups, the most tedious steps in the NGS library preparation process. The G.PURE provides a user-friendly alternative to complex automation and error-prone manual pipetting. Using an integrated dispenser and novel centrifugal evacuation technology, the product enables fast, reproducible, and tip-free bead washes and resuspension (Fig. 1).

FAQs

 

Solutions for NGS Library Construction 


Choosing the Right Workflow 

The best choice depends on your specific needs and priorities. Consider factors like: 

  • Budget: If cost is a major concern, a semi-automated approach might be more feasible. 
  • Throughput: It may seem counterintuitive, but for high-volume sequencing, a semi-automated system may be more efficient thanks to batch sample processing. 
  • Flexibility: If you need adaptability for custom projects, a semi-automated approach offers more freedom. 
  • Technical expertise: Evaluate your lab's resources and ability to manage complex automated systems. Choosing a science-minded automation partner will alleviate the need for your lab to know it all.
  • Data quality: Consider the importance of error-free, reproducible data for your research. 

Embrace the Future of Laboratory Automation

Watch as your research endeavors soar to new heights with the I.DOT Non-Contact Dispenser and G.PURE Clean-Up Device by your side. Book a demo today!