Synthetic Biology
Gene Libraries, Assembly, and Cloning

An Intersection Between Biology and Technology

Synthetic biology is an interdisciplinary field that combines principles from biology, engineering, and computer science to design and construct new biological parts, devices, and systems with enhanced or novel functions. It involves the engineering of biological components, such as DNA sequences, genes, and proteins, to create synthetic organisms or modify existing ones for specific purposes. Synthetic biologists aim to understand and manipulate biological systems at the molecular level, enabling them to engineer cells and organisms with desired traits or capabilities. Applications of synthetic biology include bioengineering, biotechnology, pharmaceuticals, agriculture, energy production, and environmental remediation. It holds promise for creating innovative solutions to address various societal and scientific challenges.

I.DOT Liquid Handling

the I.DOT Liquid Handler's precise and automated dispensing capabilities streamline and enhance various aspects of synthetic biology experiments, facilitating DNA assembly, high-throughput cloning, gene library construction, and protein engineering.

DNA Assembly

The I.DOT can accurately dispense DNA fragments, enzymes, and other reagents needed for DNA assembly methods such as PCR-based assembly, Gibson assembly, or Golden Gate assembly. It allows precise and automated dispensing of small volumes, facilitating the construction of synthetic gene circuits or genetic constructs.

High-Throughput Cloning

Synthetic biology often involves cloning and manipulating large numbers of DNA fragments. The I.DOT's high-throughput capabilities enable efficient and precise dispensing of DNA templates, vectors, and other components required for cloning processes, streamlining the workflow and reducing time and error.

Gene Library Construction

The I.DOT supports the creation of gene libraries by accurately dispensing DNA fragments into specific well locations of a plate. This capability is crucial for applications such as directed evolution, pathway engineering, or screening large numbers of genetic variants.

Protein Engineering

Synthetic biology experiments often involve protein engineering, where the I.DOT can dispense enzymes, substrates, and other protein-related components. This enables researchers to precisely control reaction conditions and optimize protein production or modification.