Publication Highlight: I.DOT for High Throughput Multi-Spheroid Formation in an Array Format

In vitro reproduction of native tissues is an important tool, as it facilitates the study of fundamental biological processes, as well as the screening of possible drugs and investigating their toxicity potential. Hence, artificial multicellular systems are created in a 3D microenvironment from different cell types in spatially organized structures or well-defined geometries to accurately resemble complex tissues in vitro.

However, in vitro reconstruction of complex tissue architectures remains challenging, thus technologies that allow for controllable and high-throughput synthesis of large multicellular architectures are required.

I.DOT has enabled the authors of the following publication in:

  • Low-volume DMEM (Dulbecco’s Modified Eagle’s Medium) media dispensing on DMA (Droplet-microarray) slides
  • Low-volume (50 to 300nl) HepG2, HeLa and HEK 293 T cell dispensing
  • Highly viable double- spheroid, homologous and heterologous multi-spheroid architectures of HepG2, HeLa and HEK 293 T cell lines by PROgrammable Merging of Adjacent Droplets method (proMAD) in a high throughput array format to investigate the propagation of Wnt signalling.

Wnt signaling is a cell-to-cell communication network that is critical for several biological processes during development and adult homeostasis, as well as disease progression, including cancer.

Thus, the authors developed a proMAD approach on droplet microarray (DMA) platform, which offers a miniaturized, high throughput, and cost-effective method for 3D cell culture as compared to existing methods.

Access the free research article for more information.

Download: https://doi.org/10.1002/adma.202006434

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