Antibodies, also known as immunoglobulins (Ig), are glycoproteins produced by differentiated B lymphocytes known as plasma cells in response to antigen susceptibility. The antibodies can then be used in a variety of applications such as western blot (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), chromatin immunoprecipitation (ChIP), and flow cytometry (FC).
In recent years, several types of antibodies, including primary and secondary antibodies, as well as monoclonal and polyclonal antibodies with varying specificities, have been developed. These advancements have made antibodies indispensable components in a wide range of applications in medicine, biomedical research, and diagnostics. The broad range of antibody-related applications is undoubtedly needed in the pursuit of scientific discoveries. However, to produce reliable and reproducible results for specific experiments, antibody-based methods consume time, labor, accuracy, and money.
The I.DOT Non-contact Dispenser and its adaptability to consumables have shown assurance in dispensing antibodies and support biomedical research and development of therapeutics. The data presented in this application note show that using I.DOT for antibody dispensing has no effect on the concentration of antibodies dispensed. As a result, the I.DOT can be used to achieve consistent and reliable results besides dispensing different sizes of antibodies quickly and accurately.
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