Compatibility of I.DOT consumables with antibodies

Compatibility of I.DOT consumables with antibodies of different sizes

Antibodies are indispensable in a wide range of applications in diverse fields of molecular biology. The superior sensitivity and specificity of antibodies makes them valuable in biomedical research and in medical diagnostics. Antibodies are key components in Western Blotting, Enzyme-linked immunosorbent assays (ELISAs), Immunoprecipitation assays, immunohistochemistry, and immunocytochemistry. Furthermore, in flow cytometry, antibodies are widely used for intracellular analysis.

Material and Method

Antibodies with different sizes (16-160kDa) and clonality (monoclonal and polyclonal) were diluted (1:2000) in the antibody diluent (BIOZOL). Next, these antibodies were dispensed by the I.DOT with different consumables (S.60, S.100, and S.200) at different pressure points ranging from 70-250 mbar*ms.

Antibodies dispensing results

After a thorough investigation of dispensing behaviours of a range of antibodies of different sizes (16-160 kDa) on different I.DOT consumables, we present an overview of I.DOT the dispensing of antibodies and how to get your settings right for dispensing differently sized antibodies for your intended applications.

Result and Summary:

We recommend using the S.200 Wells as well as restricting the pressure for the liquid class to max. 250 mbar/ms. We do not recommend using lowest and the highest-pressure points of the I.DOT settings.

Recommendations for dispensing antibodies with the I.DOT

• Use the recommended consumables and pressure settings
• Do not let the antibody solutions in the source wells sit for more than five minutes before dispensing. It could cause clogging of the wells.

Here is our flyer that you can download and use as a reference. Dispensing_different_sizes_of_Antibodies

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I.DOT

The I.DOT is a liquid handler that anyone can use regardless of automation experience. The instrument transfers volumes as low as 8 nL with unrivaled precision and speed while dramatically reducing your laboratory’s plastics and reagent consumption.

Whether you are dispensing enzymes and beads for NGS or qPCR or adding cells, compounds, and buffers for Assay Development or High-throughput Screening, the I.DOT offers unrivaled precision and ease of use for efficient and reproducible sample preparation.

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